セミナー
Kyoto Institute of Technology, Kyoto, JAPAN May 16, 2017, Rm#118, 2nd building
(Hideyuki NAKANISHI, Chair)
| 10:30-10:55 | Robert Horvath Nanobiosensorics Group, MTA EK MFA, Budapest, Hungary “Biomolecules and living cells monitored by label-free optical waveguide sensors” |
| 10:55-11:20 | Beatrix Péter Nanobiosensorics Group, MTA EK MFA, Budapest, Hungary “Living cells and copolymer coatings exposed to green tea polyphenol (EGCg): dynamic investigations using label-free optical biosensors” |
| 11:20-11:45 | Istvan Lagzi Department of Physics, Budapest University of Technology and Economics, Budapest, Hungary “Master-slave and peer-to-peer coupling in chemical systems” |
Contact: Hideyuki NAKANISHI
Tel: +81-75-724-7848, Email: hnakanis@kit.ac.jp
Robert Horvath
Nanobiosensorics Group, MTA EK MFA, Budapest, Hungary (www.nanobiosensorics.com)
Title: Biomolecules and living cells monitored by label-free optical waveguide sensors
Abstract: The present talk highlights the most recent optical waveguide based biosensors to monitor biomolecules and living cells. Our group is developing and applying such sensors for highly sensitive analyzis of surface processes in real time, without the need for any labels. These biosensors offer the possibility to monitor the binding of small molecules, the adhesion kinetics of cells, and molecular movement inside living cells; highly relevant for the development of biomedical surfaces and drug discovery.
Beatrix Péter
Nanobiosensorics Group, MTA EK MFA, Budapest, Hungary (www.nanobiosensorics.com)
Title: Living cells and copolymer coatings exposed to green tea polyphenol (EGCg): dynamic investigations using label-free optical biosensors
Abstract: Scientists examine the positive effects of epigallocatechin-gallate (EGCg), one of the active substances of green tea, for a long while. In the literature the authors mostly use labeling methods in general, thus, examination of this specific material EGCg by label-free techniques opens up new perspectives. In our work, optical label-free biosensors were applied to investigate the green tea polyphenol and its effects on living cells (HeLa cell line) and on copolymer coatings in a real-time way.
Istvan Lagzi
Department of Physics, Budapest University of Technology and Economics, Budapest, Hungary
Title: Master-slave and peer-to-peer coupling in chemical systems
Abstract: Design strategy through linking a driving pH oscillator (master system) to a pH sensitive complexation, precipitation or protonation equilibrium (slave system) has been widely used to create and control concentration oscillations of chemical entities (e.g., monovalent cations, DNA, nanoparticles) not participating in the pH oscillatory system. No systematic investigation has been carried out on how the components of these equilibria affect the characteristics of the driving pH oscillators, and this feedback effect has been often neglected in previous studies. Here we show that pH sensitive species (hydrogen carbonate, EDTA) through a pH dependent equilibrium could significantly affect the characteristics (time period and amplitude) of the driving pH oscillators. By varying the concentration of those species we are able to control the strength of the chemical feedback from slave system to master system thus introducing a transition from master-slave coupling to peer-to-peer coupling in linked chemical systems.
